Key features and details | |
Cat. No. | MABL-136 |
Name | Anti-Stonin-2 mAbs |
Clone No. | AFD-N346/9 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC, WB, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for Stonin-2 epitope: amino acids 1-555. The antibody does not cross react with stonin-1. Stonin-2 is an adapter protein involved in endocytic machinery. Involved in the synaptic vesicle recycling. May facilitate clathrin-coated vesicle uncoating. |
Alternative Name | N346/9R; STON2; Stoned B |
UniProt | Q8WXE9 |
Immunogen | This antibody was raised by immunising BALB/c mice with a fusion protein amino acids 1-555 (N-terminus) of human Stonin-2. |
Application Notes | This antibody is recommended for detection and analysis of stonin-2 by immunocytochemistry, immunohistochemistry and western blot. For instance, the mouse version of this antibody was used to detect stonin-2 by western blot in extracts of COS cells transiently transfected with HA-tagged Stonin-1, Stonin-2 or untagged Kv2.1 plasmid. Furthermore it was used to detect stonin-2 by immunocytochemistry in adult hippocampus from Stonin-2 wild-type and knockout mice. |
Antibody First Published | Andrews et al. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360 |
Note on publication | This article describes the generation of a library of recombinant monoclonal antibodies (R-mAbs) from a pool of mAb-producing hybridomas for neuroscience research. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
