Anti-Synaptotagmin-12 mAbs

Cat. No.

MABL-124

Name

Anti-Synaptotagmin-12  mAbs

Clone No.

AFD-N277/7

From

Recombinant Antibody

Isotype

Engineer antibody

Application

 ICC, WB, IHC

Species Reactivity

 Rat, Mouse

Basic Information

Specificity

This antibody is specific for the   synaptotagmin-12 protein. The antibody does not cross-react with   Synaptotagmin-6 (or other Synaptotagmins). Synaptotagmin-12 is a synaptic   vesicle phosphoprotein that enhances spontaneous neurotransmitter release but   does not effect induced neurotransmitter release (By similarity). Unlike   other synaptotagmins, it does not bind Ca2+ or phospholipids (By similarity).

Alternative Name

N277/7R; Syt12; Synaptotagmin XII; SytXII

UniProt

Q920N7

Immunogen

This antibody was raised by immunising   BALB/c mice with a fusion protein amino acids 168- 255 (cytoplasmic C2A   domain) of mouse Synaptotagmin-12

Application Notes

This antibody is recommended for the   detection and analysis of Synaptotagmin-12 by immunocytochemistry,   immunohistochemistry and western blot. For instance, the mouse version of   this antibody was used to detect Synaptotagmin-12 by western blot in extracts   of COS cells transiently transfected with GFP-tagged Synaptotagmin plasmid.

Antibody First Published

Andrews et al. A toolbox of IgG   subclass-switched recombinant monoclonal antibodies for enhanced multiplex   immunolabeling of brain eLife. 2019; 8: e43322. PMID:30667360

Note on publication

This article describes the generation of a   library of recombinant monoclonal antibodies (R-mAbs) from a pool of   mAb-producing hybridomas for neuroscience research.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.