Anti-Tau mAbs

Cat. No.

MABL-049

Name

Anti-Tau  mAbs

Clone No.

AFD-PC1C6

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, IHC

Species Reactivity

Bovine, Rat, Human

Basic Information

Specificity

This antibody was generated against purified   denatured bovine microtubule associated proteins (MAPs) and recognizes human,   rat, and bovine Tau1. Tau protein is a highly soluble microtubule- associated   protein. In humans, these proteins are mostly found in neurons compared to non-neuronal cells. One of tau's main functions is to modulate the stability   of axonal microtubules. The protein interacts with tubulin to stabilize   microtubules and promote tubulin assembly into microtubules. When tau   proteins are defective, and no longer stabilize microtubules properly, they   can result in dementias such as Alzheimer's disease.

Alternative Name

TAU; microtubule associated protein tau; Tau   proteins; G protein beta1/gamma2 subunit-interacting factor 1; MAPT;   Microtubule-associated protein tau; MTBT1; Neurofibrillary tangle protein;   Paired helical filament-tau; PHF-tau; Tau microtubule-associated protein;   Tau1; Tau-1; Tau 1

UniProt

P10636

Immunogen

This antibody was raised by immunising   BALB/c mouse with purified denatured bovine microtubule associated proteins.

Application Notes

In the original study, this antibody was used in immunoblot assays and competitive ELISA to show that tau was more   abundant in bovine white matter extracts and microtubules than in extracts and microtubules from an enriched gray matter region of the brain (Binder et   al., 1985). Example of functional studies in which this antibody could be utilised include those conducted by Kosik et al. (PNAS 1986) to confirm that   tau is a major antigenic component of paired helical filaments in Alzheimer   disease, by Bramblett et al. (Neuron 1993) to demonstrate that abnormal tau   phosphorylation at Ser396 in Alzheimer's disease recapitulates development   and contributes to reduced microtubule binding, by Stamer et al. (J Cell   Biol. 2002) to suggests a linkage between tau and amyloid precursor protein   (APP) trafficking, and by Strang et al. (JBC 2018) to provide novel insights   in the molecular mechanisms of tau aggregation.

Antibody First Published

Binder et al. The distribution of Tau in mammalian central nervous system. J Cell Biol. 101(4):1371-8 (1985).   PMID:3930508

Note on publication

Describes the original generation of this antibody and its subsequent applications to study the distribution of   tau in the mammalian central nervous system.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.