Key features and details | |
Cat. No. | MABL-048 |
Name | Anti-GABA-A Receptor, Gamma 2 Subunit mAbs |
Clone No. | AFD-10F10-C1-B8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IF, IHC |
Species Reactivity | Rat |
Basic Information | |
Specificity | This antibody was generated against amino acids 37-53 of rat GABA-A receptor gamma2 subunit (GABRG2) and is specific for the N-terminal extracellular domain, allowing for the detection of surface expressed receptors for live imaging. GABA-A receptors are ligand-gated neurotransmitter receptors that function to mediate the fast synaptic inhibition within the brain. They are pentamers, composed from a variety of classes of subunits with gamma being just one class. Each subunit is composed of an extracellular N-terminal domain, four membrane spanning units, and a small extracellular C-terminal segment. Several mutations in the GABA(A) receptor subunit gamma-2 expression have been linked to epileptic syndromes that result in fibrile seizures. |
Alternative Name | GABARG2; GABAR-G2 CAE2; ECA2; GABA(A) receptor subunit gamma-2; GABA(A) receptor, gamma 2; gamma 2; gamma-aminobutyric acid (GABA) A receptor, gamma 2; Gamma- aminobutyric acid receptor subunit gamma-2; GEFSP3; 10F10C1B8; 10F10.C1.B8 |
UniProt | P18508 |
Immunogen | This antibody was generated in mouse against the gamma2 subunit of the GABA-A protein from rats. |
Application Notes | In the original study, this antibody was used in Western Blot assays, immunohistochemistry (IHC) and immunoprecipitate (IP) for identification of the γ2 subunit from rat and mouse hippocampal proteins (Joshi et al., 2011). This antibody could also be used in functional studies, for example, to show that the GABA-A receptor membrane insertion rates are specified by their subunit composition (Joshi et al, 2013), to delineate how chorein deficiency leads to upregulation of gephyrin and GABAA receptor (Kurano et al., 2006), to examine the homeostatic strengthening of inhibitory synapses as mediated by the accumulation of GABAA receptors (Rannals et al., 2011), and to demonstrate that NMDA receptor activation downregulates expression of δ Subunit-containing GABA-A receptors in cultured hippocampal neurons (Joshi et al. 2013). |
Antibody First Published | Joshi et al. A mouse monoclonal antibody against the gamma2 subunit of GABA-A receptors. Hybridoma. 30(6): 537-42 (2011). PMID:22149279 |
Note on publication | Describes the original generation and characterisation of this antibody, which was raised against the gamma2 subunit of rat GABA-A receptors |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
