Key features and details | |
Cat. No. | MABL-039 |
Name | Anti-disialoganglioside 2 mAbs |
Clone No. | AFD-7A4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Depletion, RIA, TLC, ELISA, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | 7A4 binds to GD2: GD2+ neuroblastoma cell lines and melanomas, small-cell lung carcinomas, rhabdomyosarcomas, Ewings sarcomas, Wilm's tumors and retinoblastomas also expressing GD2. GD2- cell lines or GD2- peripheral blood cells are not bound by the antibody. The antibody and the scFv cross-react weakly with GD3 and GM2. GD2 is a target for tumor therapy due to its high level of expression in the majority of neuroblastoma and melanoma cells, and poor levels of expression in normal tissues. |
Alternative Name | GD2 |
UniProt | |
Immunogen | 7A4 was generated by hyperimmunizing mice with recombinant gamma-interferon-treated LAN-1 neuroblastoma cells. |
Application Notes | 7A4 is able to lyse tumour cells by complement-mediated cytotoxicity (lytic dose ~10 ng) and can perform in vitro direct binding of labelled mAb to living and glutaraldehyde-fixed cells. Shows strong binding to all neuroblastomas tested in addition to other GD2-expressing cells such as melanomas, small-cell lung carcinomas, rhabdomyosarcomas, Ewings sarcomas, Wilm's tumors and retinoblastomas.TLC analysis can be used to confirm the antibody target antigen. The 7A4 scFv possesses the same specificity as the full-length antibody but slightly weaker binding affinity. The scFv has been used in IF studies (PMID: 9309424). |
Antibody First Published | Gross et al. New anti-GD2 monoclonal antibodies produced from gamma- interferon-treated neuroblastoma cells International journal of cancer, 15 April 1989, Vol.43(4), pp.665-71 PMID:2467885 |
Note on publication | Describes the characterization of 7A4 and other anti-neuroblastoma antibodies by TLC, IHC, in vitro direct binding, complement-cytotoxicity assays and solid phase RIA. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
