Anti-Crotoxin mAbs

Cat. No.

MABL-038

Name

Anti-Crotoxin mAbs

Clone No.

AFD-CTX-scFv8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

neutralize, ELISA

Species Reactivity

South-American rattlesnake Crotalus   durissus terrificus

Basic Information

Specificity

scFv binds to crotoxin and   recognises epitopes overlapping BI-a (an epitopic region at the interface   between the CA and CB subunits of the heterodimer) and BIII (an epitopic   region on CB involved in the enzymatic activity of CB) - scFv 8 may be able   to caused the two subunits to dissociate and suppress the synergistic   activity between them. Crotoxin is a phospholipase A2 neurotoxin and is the   main toxic component in venome from Crotalus durissus terrificus   rattlesnakes, acting by blocking neuromuscular transmission.

Alternative Name

Phospholipase A2 crotoxin basic   chain; CB; svPLA2; Cdcum6;Phosphatidylcholine 2-acylhydrolase

UniProt

P24027

Immunogen

8 was constructed from a phage   display library using V-genes from 43 non-immunized donors.

Application Notes

scFv 8 is able to reduce the   myotoxicity induced by crotoxin and is able to neutralize the lethality   induced by the toxin. This scFv was also used for (competitive ELISAs).

Antibody First Published

Cardoso et al. Neutralizing human   anti crotoxin scFv isolated from a nonimmunized phage library. Scand J   Immunol. 2000 Apr;51(4):337-44. PMID:10736105

Note on publication

Describes the construction and a phage   library using V-genes from non-immunizzed donors. Studies were conducted   which determined the binding region of the scFv and the neutralizing effect   of the scFv on mycotoxicity and lethality in mice.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.