Cat. No.
MABL-3738
Application
Blocking, WB, FC, IF
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
TAN1-6
From
Recombinant Antibody
Specificity
This antibody is specific for murine CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Alternative Names
TNFSF7; CD27 ligand; CD27-L; Cd27l; CD27LG; CD70 antigen; Ki-24 antigen; Tnfsf7; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7
UniProt
O55237
Immunogen
This antibody was raised by immunising LOU rats with soluble recombinant CD70:Fc fusion protein
Application Notes
This antibody has been used in various FACS analyses to reveal a critical role for CD70 in CD8 T cell priming by CD40-licensed antigen-presenting cells (Taraban et al, 2004), to demonstrate the differential requirement for CD70 and CD80/CD86 in dendritic cell-mediated activation of tumor-tolerized CD8 T cells (Bak et al, 2012), to examine how temporal changes in dendritic cell subsets, cross-priming and costimulation via CD70 control CD8(+) T cell responses to influenza (Ballesteros-Tato et al, 2010), and to show that CD70 is downregulated by interaction with CD27 (Kuka et al, 2013). In addition, this antibody has been used in vivo to block the CD70-CD27 interaction as part of the two studies examining how CD70 is critically required for the priming of CD8+ T cells (Taraban et al, 2004; Taraban et al, 2006).
Antibody First Published
Taraban Vadim et al. Cutting edge: a critical role for CD70 in CD8 T cell priming by CD40-licensed APCs. J Immunol. 2004 Dec 1;173(11):6542-6. PMID:15557144
Note on publication
Describe the original generation of this antibody and its subsequent use in FACS and in vivo experiments to demonstrate the critical role for CD70 in CD8 T cell priming by CD40-licensed antigen-presenting cells.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
