Cat. No.
MABL-3734
Application
CoStim, SPR, Superagonism, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
D665
From
Recombinant Antibody
Specificity
This antibody binds to the laterally exposed C′D loop of CD28 and induces T cell proliferation without TCR ligation.
Alternative Names
TP44; T-cell-specific surface glycoprotein CD28; TP44
UniProt
P31041
Immunogen
The original D665 antibody was generated by immunizing mice alternately with A20 cells expressing mCD28 i.p. and with recombinant mCD28Ig s.c.
Application Notes
This antibody binds to mouse CD28. The original mouse IgG1 version was shown to trigger T-cell proliferation in vitro and in vivo in a TCR-independent manner and is a superagonistic antibody. This antibody does not compete with E18 (Ab00286).
Antibody First Published
Dennehy et al. Cutting Edge: Monovalency of CD28 Maintains the Antigen Dependence of T Cell Costimulatory Responses Journal of Immunology PMID:16670276
Note on publication
Cutting Edge: Monovalency of CD28 Maintains the Antigen Dependence of T Cell Costimulatory Responses
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
