Cat. No.
MABL-3732
Application
Activating, IP, FC, IF
Isotype
Engineer antibody
Species Reactivity
Rat, Human, Mouse
Clone No.
LG.3A10
From
Recombinant Antibody
Specificity
This antibody is specific for mouse CD27, which, as in the human system, is largely restricted to cells of the T cell lineage, and is expressed on the great majority of both αβ and γδ T lymphocytes, and thymocytes.
Alternative Names
TNFRS7; CD27L receptor; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7
UniProt
P41272
Immunogen
This antibody was produced by injecting Armenian hamsters intraperitoneally with a mixture of two murine CD27 tag-expressing Armenian hamster fibroblast clones (AR0524 and AR0530) in Freund's adjuvant
Application Notes
This antibody is specific for murine CD27, and has been used to assess the distribution of CD27 expression using IF, FC and IP analysis (Gravstein, 1995). Upon cross-linking, this antibody has been shown to induce a 4-fold increase in T cell proliferation in response to suboptimal stimulation with concanavalin A (Gravestein, 1995), indicating that this antibody is able to mimic ligand binding.
Antibody First Published
Gravstein et al. Novel mAbs reveal potent co-stimulatory activity of murine CD27 International immunology, April 1995, Vol.7(4), pp.551-7 PMID:7547681
Note on publication
Describes the original use of this antibody to elucidate the distribution and co- stimulatory activity of CD27.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
