Cat. No.
MABL-3726
Application
FACS, IP, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
JOVI.1
From
Recombinant Antibody
Specificity
This antibody binds to human V-beta 3 chain of the T-cell receptor. In particular this antibody binds to the TRBC1 variant but not the TRBC2 variant.
Alternative Names
V(beta)3 T cell receptor (TcR)
UniProt
Immunogen
Thymus, spleen and mesenteric lymph nodes isolated from a mouse transgenic for human Vb3 TcR.
Application Notes
This antibody can be used to detect the TCR beta chain in a majority (50-75%) of T-cell receptors by flow cytometry, immunofluorescence and can immunoprecipitate the TCR beta protein (variant 1). Recently it has been combined into a chimeric antigen antigen receptor therapy approach to selectively deplete a subset of T-cells in T-cell lymphomas or leukaemias, whilst leaving the non-reactive T-cell subset intact.
Antibody First Published
Viney et al. Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice. Hybridoma. 1992 Dec;11(6):701-13 PMID:1284120
Note on publication
Describes the generation and initial characterisation of the reactivity of this antibody clone.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
