Cat. No.
MABL-3723
Application
Blocking, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
D2E7 (Adalimumab)
From
Recombinant Antibody
Specificity
Binds to soluble TNF-alpha, but not to lymphotoxin (TNF beta).
Alternative Names
Tumor necrosis factor alpha
UniProt
P01375
Immunogen
This antibody was generated by affinity maturation from the 2SD4 scFv, which in turn was selected on human TNF alpha by guided phage-display technology, using the murine anti-hTNF alpha antibody Mab32 as a template (c.f. also US Patent 6090382).
Application Notes
Adalimumab binds specifically to TNF and neutralizes the biological function of TNF by blocking its interaction with the p55 and p75 cell surface TNF receptors. Adalimumab, unlike Infliximab (Ab00146), was shown to be cross-reactive with mouse TNF alpha (Arnoult 2017, PMID: 28584008).
Antibody First Published
Jespers et al., Guiding the selection of human antibodies from phage display repertoires to a single epitope of an antigen. Nature Biotechnology 12, 899 - 903 (1994) PMID:7521646
Note on publication
The original publication describes the generation of this antibody by guided phage- display.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
