Cat. No.
MABL-3688
Application
WB, Block, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
hu5c8 (Ruplizumab)
From
Recombinant Antibody
Specificity
The antibody binds to CD40L with an IC50 of 0.845 ug/ml.
Alternative Names
CD154; CD40 ligand; tumour necrosis factor ligand superfamily member 5; TNFSF5; tumour necrosis factor related activation protein; TRAP
UniProt
P29965
Immunogen
Human CD40L
Application Notes
The antibody binds specifically to CD40L, a surface receptor expressed on activated T cells which acts as a costimulatory molecule to trigger immune responses. The antibody neutralises CD40L function, as it blocks the interaction between CD40 and CD40L. When injected into cynomolgus monkeys, the original therapeutic antibody was found to have a T1/2 of 531 ± 155 hours.
Antibody First Published
Karpusas et al. Structure of CD40 Ligand in Complex with the Fab Fragment of a Neutralizing Humanized Antibody Structure. 2001 Apr 4;9(4):321-9. PMID:11525169
Note on publication
Describes the role of CD40-CD40L interaction in immune responses and pathologies. Also describes the generation of humanized monoclonal antibodies against CD40L and the use of a crystal structure to determine its binding.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
