Cat. No.
MABL-3683
Application
FC, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
hP67.6 (Gemtuzumab)
From
Recombinant Antibody
Specificity
Recognises human CD33.
Alternative Names
Siglec-3
UniProt
P20138
Immunogen
Gemtuzumab was generated by huminization of the murine P67.6 clone, raised against CD33- transfected FMY9S5 cells of human origin. CD33 is a transmembrane receptor expressed on predominantly on cells of the myeloid lineage, but it can also be found on some lymphoid cells.
Application Notes
A human IgG4 variant conjugated to derivative of the cytotoxic drug calicheamicin gamma 1, N-acetylcalicheamicin dimethyl hydrazide, has been used as a therapeutic against acute myeloid leukemia. This antibody may also be used in flow cytometry and immunofluorescence.
Antibody First Published
Appelbaum et al. The use of radiolabeled anti-CD33 antibody to augment marrow irradiation prior to marrow transplantation for acute myelogenous leukemia. Transplantation PMID:1440849
Note on publication
Describes the use of the parental antibody conjugated to a radionucleotide in marrow irradiation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
