Cat. No.
MAK-0106
Product Name
Chicken IgG
Storage
4°C
Species
Chicken
Instructions
The chicken IgG ELISA kit is intended for measurement of IgG in serum, plasma, and egg-yolk extracts.
Introduction
The chicken IgG ELISA kit is intended for measurement of IgG in serum, plasma, and egg-yolk extracts. Goat anti-chicken IgG (H+L) antibodies are used for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-chicken IgG (Fc specific) antibodies are used for detection. Cross-reactivity with chicken IgM is ≤1%. IgM does not interfere with quantitative measurement of IgG in normal serum or plasma. Cross-reactivity with chicken IgA has not been investigated.
Normal chicken IgG levels are in the range of 3-6 mg/ml in serum or plasma and 1-2 mg/ml in egg yolk.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside prepared chicken IgG standards. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. IgG molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of IgG is proportional to the optical density of the test sample and is derived from a standard curve.
