Cat. No.
MAK-0105
Product Name
Chicken IgA
Storage
4°C
Species
Chicken
Instructions
This ELISA kit is designed for measurement of IgA in chicken serum or plasma.
Introduction
This ELISA kit is designed for measurement of IgA in chicken serum or plasma. The assay uses goat anti-chicken IgA for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated goat anti-chicken IgA antibodies for detection. Both capture and detection antibodies react specifically with chicken IgA. Cross-reactivity with immunoglobulins from other species has not been investigated.
Principle Of The Assay
Test samples are diluted and incubated in the microtiter wells for 45 minutes alongside chicken IgA standards. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. IgA molecules are thus sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of IgA is proportional to the optical density of the test sample and is derived from a standard curve.
