Cat. No.
MAK-0147
Product Name
Dog CRP
Storage
4°C
Species
Dog
Introduction
CRP is an acute phase protein that is elevated in serum due to injury, infection, pregnancy or disease. Levels of CRP in dogs have been reported in the range of 1-200 µg/ml with elevations of approximately 10-fold during pregnancy and up to 100-fold during infection. Measurement of CRP provides a convenient biomarker of inflammation and disease in dogs.
Principle Of The Assay
The assay uses affinity purified dog CRP antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated dog CRP antibodies for detection. Standards and diluted
samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in CRP molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-conjugate and TMB is added and incubated for 20 minutes. If CRP is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of CRP is proportional to absorbance and is derived from a standard curve.
