Cat. No.
MAK-0146
Product Name
Monkey CRP
Storage
4°C
Species
Monkey
Introduction
CRP is an acute phase protein in monkeys that is elevated in serum because of injury, infection, or disease. Normal levels of CRP range from 0-8.3 µg/ml and levels may increase 100-fold or more during the acute phase response.
Principle Of The Assay
The assay uses affinity purified monkey CRP antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated monkey CRP antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in CRP molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRPconjugate. TMB is added and incubated for 20 minutes. If CRP is present a blue color develops. Color development is stopped by the addition of Stop solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of CRP is proportional to absorbance and is derived from a standard curve.
