Cat. No.
MAK-0144
Product Name
Mouse CRP
Storage
4°C
Species
Mouse
Introduction
CRP is an acute phase protein in that is elevated in serum due injury, infection or disease.Measurement of CRP provides a convenient marker of inflammation and disease in mice.
Principle Of The Assay
The assay uses affinity purified mouse CRP antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated mouse CRP antibodies for detection. Standards and diluted samples are incubated in the microtiter wells for 45 minutes. The wells are subsequently washed. HRP conjugate is added and incubated for 45 minutes. This results in CRP molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRPconjugate and TMB is added and incubated for 20 minutes. If CRP is present a blue color develops. Color development is stopped by the addition of Stop solution; changing the color to yellow, and absorbance is measured at 450 nm. The concentration of CRP is proportional to absorbance and is derived from a standard curve.
