Cat. No.
MAK-0143
Product Name
Trout/Salmon C1q-LP3
Storage
4°C
Species
Trout/Salmon
Introduction
C1q-like protein 3 (C1q-LP3) was identified in proteomic studies as a protein that is increased in rainbow trout and Atlantic salmon plasma during bacterial infections. Levels in healthy fish are ~ 0.1 ug/ml, increasing up to 70 ug/ml during infection.
Principle Of The Assay
The assay uses a polyclonal antibody generated against recombinant rainbow trout C1Q-LP3. It recognizes rainbow trout and Atlantic salmon C1qLP3. The unconjugated antibody is coated on wells of a microtiter plate and used for capture. A horseradish peroxidase (HRP) conjugate is used for detection. Standards and diluted samples (100 ul) are incubated in the antibody coated microtiter wells for 45 minutes. After washing the wells, HRP-conjugate (100 ul) is added and incubated for 45 minutes. If C1q-LP3 molecules are present, they are sandwiched between the capture and detection antibodies. The wells are then washed to remove unbound HRP-conjugate. TMB is added and incubated for 20 minutes. If C1q-LP3 is present, a blue color develops. Color development is stopped by addition of Stop Solution, changing the color to yellow. Absorbance is measured at 450 nm. The concentration of C1q-LP3 is proportional to absorbance and is derived from a standard curve.
