Cat. No.
MABL-3408
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AFD-C11
From
Recombinant Antibody
Specificity
This antibody binds soluble form of human ST2 which is a member of the interleukin (IL)-1 receptor family. IL-33 is the ligand for ST2, which is involved in reducing fibrosis and hypertrophy in mechanically strained tissues. In in vitro and in vivo models, ST2L transduces the effects of IL-33, while excess sST2 or abnormalities in ST2 signaling leads to cardiac hypertrophy, fibrosis, and ventricular dysfunction. Circulating soluble ST2 concentration is believed to reflect cardiovascular stress and fibrosis. ST2 is a new cardiovascular biomarker for assessing acute heart failure and predicting the outcome of chronic heart failure.
Alternative Names
ST2; IL1RL1; IL-1RL1; DER4; T1; IL-33Rα; Interleukin-1 receptor-like 1; Protein ST2; Growth Stimulation Expressed Gene 2; suppression of tumorigenicity 2
UniProt
Q01638
Immunogen
The original antibody was generated by immunizing BALB/c mice with human ST2 protein.
Application Notes
The original IgG antibody binds human ST2 recombinant protein with a binding affinity of Kd= 0.5 X 10^(-10)M. This antibody was used as a capture antibody in combination with HRP labelled D12 as detection antibody for the development of a double-antibody sandwich ELISA for detection of soluble ST2 in blood samples (CN117024595).
Antibody First Published
Note on publication
Describes the generation and characterization of antibodies against Domain I and III of human ST2L.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
