Cat. No.
MABL-3407
Application
functional assay, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human, Cynomolgus Monkey
Clone No.
AFD-ST2M51
From
Recombinant Antibody
Specificity
This antibody specifically binds an epitope in domain III of the human ST2L and cross reacts with cynomolgus monkey ST2L. ST2L a transmembrane receptor that belongs to the IL-1 receptor family which is expressed on the cell surface of a wide variety of immune cells including T cell, NK/NKT cells, basophils, eosinophils, mast cells and the newly-described non-B/non-T innate lymphoid type 2 cells, nuocytes, and natural helper cells. ST2L expression is also inducible on dendritic cells (DCs), macrophages, and neutrophils. ST2L is able to downregulate the responsiveness of Toll-like Receptors TLR2, TLR4, and TLR9, but also induce type 2 cytokine release via activation by its ligand IL-33 and association with accessory protein IL-1RAcP.
Alternative Names
ST2; IL1RL1; IL-1RL1; DER4; T1; IL-33Rα; Interleukin-1 receptor-like 1; Protein ST2; Growth Stimulation Expressed Gene 2; suppression of tumorigenicity 2
UniProt
Q01638
Immunogen
The original antibody was isolated from a human Fab phage display library by panning against hST2L ECD (residues 18–328)-Fc fusion protein. Later on the antibody was converted to a human IgG2 format.
Application Notes
This antibody is not capable of blocking the interaction between IL-33 and ST2L. This antibody was capable of recognizing recombinant human ST2L extracellular domain (ST2L-ECD) using an ELISA assay (PMID: 27294560). The original human IgG2 chimeric version of the antibody binds human and cynomolgus monkey ST2L with a binding affinity of 38 pM and 76 pM respectively (US9090694). This antibody was capable of binding ST2L expressed by LAD2 mast cells in a flow cytometric assay. This antibody was reported to block the IL-33 induced activation of NF-kB in a dose dependent manner. This antibody also blocked IL-1RAcP from binding to the ST2L/IL-33 complex. It can also inhibit cytokine secretion by human basophilic cell line KU812 (PMID: 27294560).
Antibody First Published
Fursov et al. Monoclonal antibodies targeting ST2L Domain 1 or Domain 3 differentially modulate IL-33-induced cytokine release by human mast cell and basophilic cell lines. Mol Immunol. 2016 Jul:75:178-87. PMID:27294560
Note on publication
Describes the generation and characterization of antibodies against Domain I and III of human ST2L.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
