Cat. No.
MABL-3376
Application
AlphaLISA, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
barley, rye, spelt, triticale, Wheat
Clone No.
AFD-1E01
From
Recombinant Antibody
Specificity
This antibody can recognize and bind the 'QPEQPFP' peptide of gliadins. This antibody also reacts with deaminated 33mer peptide of α-gliadin 'LQLQPFPQPELPYPQPELPYPQPELPYPQPQPF '.
Alternative Names
gluten; Celiac Allergen; Coeliac Allergen
UniProt
Immunogen
The original antibody was isolated from the IgA+ plasma cells of celiac patients gut lesions that are reactive with deaminated gluten proteins.
Application Notes
The binding specificity of IgA+ plasma cells in the single cell suspension was determined by flow cytometry. The relative affinity of the antibody to a panel of gliadin was investigated using ELISA and AlphaLISA (PMID: 27146306, WO2015164615). This antibody recognizes a sequence that intimately associates with deamidation of the gluten peptide. (PMID:28878138).
Antibody First Published
Dorum et al. Gluten-specific antibodies of celiac disease gut plasma cells recognize long proteolytic fragments that typically harbor T-cell epitopes. Sci Rep. (2016); 6: 25565.PMID:27146306
Note on publication
Describes the generation and epitope characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
