Cat. No.
MABL-3369
Application
IP, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Aspergillus fumigatus (Neosartorya fumigata)
Clone No.
AFD-17A5
From
Recombinant Antibody
Specificity
This antibody recognises Asp f 1.
Alternative Names
A. fumigatus Allergen; Asp f1; Aspf1; ribotoxin Asp f 1; ribonuclease mitogillin; allergen Asp f I; Asp f I; IgE-binding ribotoxin; major allergen Asp f 1; allergen: Asp f 1
UniProt
P67875
Immunogen
17A5 antibody was raised using a novel technique: mononuclear cells were taken from the human subjects with allergic bronchopulmonary aspergillosis and then screened for IgE secretion.Subsequently, they were used to generate hybridomas of which some were secreting anti-Asp f 1 IgE.
Application Notes
17A5 antibody's target protein (Asp f 1) was confirmed through IP and mass spectrometry analysis (Wurth et al., 2018). WB and ELISA: the antibody was successfull in detecting Asp f 1 antigen in a commercial Aspergillus extract ALKB (Wurth et al., 2018).
Antibody First Published
Wurth et al. Human IgE mAbs define variability in commercial Aspergillus extract allergen composition JCI Insight. 2018 Oct 18;3(20). pii: 123387. doi: 10.1172/jci.insight.123387. PMID:30333320
Note on publication
The paper describes a method of generating Aspergillus-reactive IgE–secreting human hybridomas (among them these secreting this antibody). Subsequently, the antibody is characterized and its specificity is determined. Finally, this antibody is used to determin a potential presence of its antigen in the commercialy-available Aspergillus samples used in diagnosis and treatment of Aspergillus-related allergies.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
