Cat. No.
MABL-3368
Application
IP, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Aspergillus fumigatus (Neosartorya fumigata)
Clone No.
AFD-4F8
From
Recombinant Antibody
Specificity
This antibody recognises Asp f 1.
Alternative Names
A. fumigatus Allergen; Asp f1; Aspf1; ribotoxin Asp f 1; ribonuclease mitogillin; allergen Asp f I; Asp f I; IgE-binding ribotoxin; major allergen Asp f 1; allergen: Asp f 1
UniProt
P67875
Immunogen
4F8 antibody was raised using a novel technique: mononuclear cells were taken from the human subjects with allergic bronchopulmonary aspergillosis and then screened for IgE secretion.Subsequently, they were used to generate hybridomas of which some were secreting anti-Asp f 1 IgE
Application Notes
This antibody's target protein (Asp f 1) was confirmed through IP and mass spectrometry analysis (Wurth et al., 2018). WB and ELISA: the antibody was successfull in detecting Asp f 1 antigen in a commercial Aspergillus extract ALKB in ELISA and western blot (Wurth et al., 2018).
Antibody First Published
Wurth et al. Human IgE mAbs define variability in commercial Aspergillus extract allergen composition JCI Insight. 2018 Oct 18;3(20). pii: 123387. doi: 10.1172/jci.insight.123387. PMID:30333320
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
