Cat. No.
MABL-3363
Application
chimera studies, SPR
Isotype
Engineer antibody
Species Reactivity
Dermatophagoides pteronyssinus Immunogen
Clone No.
AFD-H5
From
Recombinant Antibody
Specificity
H5 binds a distinct epitope to clones H10 and H3.
Alternative Names
Mite Allergen; Mite group 2 allergen Der p 2; Allergen Der p II; DPX
UniProt
P49278
Immunogen
The antibody was generated by immunising mice with Der p 2.
Application Notes
The binding of chimeric H5 to Der p 2 was confirmed using SPR, with a Kd value of approximately 30 nM. SPR also showed it binds distinct epitopes to clones H3 and H10 (Christensen et al., 2008; PMID: 18572230).
Antibody First Published
Christensen et al. Several distinct properties of the IgE repertoire determine effector cell degranulation in response to allergen challenge J Allergy Clin Immunol. 2008 Aug;122(2):298- 304. PMID:18572230
Note on publication
Descibes the original production of the antibody and its characterisation through SPR.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
