Cat. No.
MABL-3361
Application
deplete, IP, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
AFD-Mar-1
From
Recombinant Antibody
Specificity
Holzmann et al. A model system using tape stripping for characterization of Langerhans cell-precursors in vivo. J Invest Dermatol. 2004 May;122(5)
Alternative Names
Fcer1a; Fc epsilon RI alpha; FceRIa; FceRI alpha; FceRI-α; high affinity IgE receptor; High affinity immunoglobulin epsilon receptor subunit alpha;Fce1a; Fc-epsilon RI-alpha; FcERI; IgE Fc receptor subunit alpha
UniProt
P20489
Immunogen
Application Notes
This antibody has been used in flow cytometric analysis of FceR1 alpha cell surface expression by basophils (Min et al, 2004; Chen et al, 2009), and immunohistochemical analysis of epidermal sheets (Holzmann et al, 2004). It has also been used in immunoprecipitation analysis of cell lysates from lung cDCs and MC/9 mast cells (Grayson et al, 2007). In vitro incubation of basophils with this antibody blocks subsequent MAR-1 staining, but does not activate basophil IL-4 production (Sokol et al, 2008). Intravenous treatment of mice with this antibody results in basophil depletion in peripheral blood, spleen, bone marrow and liver, but not skin or intraperitoneal mast cell depletion (Sokol et al, 2008).Following treatment with this antibody, basophil-depleted mice display impaired Th2 differentiation upon papain immunisation (Sokol et al, 2008). However, MAR-1 treated mice respond normally to high-dose intravenous Ig in both the K/BxN serum transfer arthritis and collagen-induced arthritis models, despite basophil depletion (Campbell et al, 2014).
Antibody First Published
Holzmann et al. A model system using tape stripping for characterization of Langerhans cell-precursors in vivo. J Invest Dermatol. 2004 May;122(5):1165-74. PMID:15140219
Note on publication
Describes the original generation of this antibody, and its characterisation in ELISA and FC analysis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
