Cat. No.
MABL-3598
Application
WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
SARS-CoV, SARS-CoV-2
Clone No.
1C7C7
From
Recombinant Antibody
Specificity
This antibody specifically targets an epitope on the SARS-CoV-2 and SARS-CoV nucleocapsid protein and does not cross-react with the nucleocapsid of other common human coronaviruses, OC43 and 229E.
Alternative Names
nucleoprotein; NP; NC; Protein N; Nucleocapsid protein; SARS-CoV Protein N; SARS-CoV-2 Nucleocapsid protein; SARS Coronavirus; SARS-CoV-2; SARS CoV 2; 2019-nCoV; 1C7; severe acute respiratory syndrome virus
UniProt
P0DTC9
Immunogen
The original version of this antibody was raised against SARS-CoV-1/2.
Application Notes
The antibody 1C7C7 detects SARS-CoV-2 and SARS-CoV-1 and can be used in affinity binding assays, flow cytometry, immunocytochemistry, and western blotting. Its effectiveness in detecting the nucleocapsid of the novel coronavirus causing COVID-19 was confirmed in multiple studies. For instance, it was used to analyze SARS-CoV-2 nucleoprotein extracted from infected cells via Western blot (Hoagland et al., 2020) ,and similarly by Blanco-Melo et al. in a study on imbalanced host response to SARS- CoV-2 (2020; pmid: 32416070). Another group has used this clone to stain infected cells fixed with 10% neutral formalin during a neutralization assay (Piepenbrink et al., 2020). Daniloski et al. performed similar staining procedure with 1C7C7 for a trans-complementation assay (2020; pmid: 33147445). Finally, this antibody was also used to stain SARs-CoV-2 infected cells in a study on in vitro susceptibility to SARS-CoV-2 infection (Dobrindt et al., 2020; pmid: 32995783).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
