Cat. No.
MABL-3597
Application
biolayerinterferometry, NTRL, ELISA
Isotype
Engineer antibody
Species Reactivity
SARSCoronavirus2 (SARS-Cov-2)
Clone No.
Sb23
From
Recombinant Antibody
Specificity
This antibody is capable of binding both recombinant RBD as well as to the pre-fusion spike glycoprotein with high affinity. Sb23 binds an epitope in the ACE2 binding site on the RBD in both its ‘up’ and ‘down’ conformation and thereby effectively blocks ACE2 binding.
Alternative Names
SARS CoV 2 S glycoprotein; Sb23; COVID-19 Spike protein; RBD; Receptor Binding Domain; E2 glycoprotein; E2; Human coronavirus 2 spike glycoprotein; Peplomer protein; S glycoprotein; SARS coronavirus 2 S protein; SARS coronavirus 2 Spike Protein; SARS CoV 2 Spike protein; SARS CoV 2; SARS-CoV-2 S protein; SARSCoV2; SARS-COV-2 S protein; SARS-COV-2 Spike glycoprotein; SARSCOV2 Spike protein; Severe acute respiratory syndrome 2 spike glycoprotein; Severe acute respiratory syndrome virus 2 spike glycoprotein; Spike glycoprotein; 2019-nCoV
UniProt
P0DTC2
Immunogen
The original antibody was
Application Notes
Sb23 binds RBD with a very high affinity and neutralizes pseudovirus with an IC50 of 0.6 µg/ml. Sb23 and ACE2 compete for the same or overlapping binding sites on SARS-CoV-2-RBD. ELISA was used to screen the libraries to identify specific RBD binders. It is also reported that since Sb23 can efficiently replace ACE2 from a preformed complex, it has a higher affinity for RBD than ACE2. Biolayer interferometry assay was used to kinetically characterize the interaction of sybodies with RBD and spike (Custódio et al., 2020).
Antibody First Published
Custódio et al. Selection, biophysical and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 . BioRxiv (2020)
Note on publication
Describes the isolation, characterization and structural analysis of synthetic nanobodies that effectively neutralize SARS-CoV-2 .
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
