Key features and details | |
Cat. No. | MABL-3437 |
Name | Anti-MBP mAbs [MBP-7128](MABL-3437) |
Clone No. | MBP-7128 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA |
Species Reactivity | Escherichia coli |
Basic Information | |
Specificity | This antibody recognizes and binds maltose binding protein of E. coli. |
Alternative Name | malE; MMBP; Maltose-binding periplasmic protein; Maltodextrin-binding protein |
UniProt | P0AEX9 |
Immunogen | The original antibody was generated as a diabody by fusing two different Fv domains by short peptide linkers. |
Application Notes | Maltose-binding protein (MBP) is a part of the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. Maltose- binding protein (MBP) is one of the most popular fusion partners being used for producing recombinant proteins in bacterial cells. MBP allows one to use a simple capture affinity step on amylose-agarose columns, resulting in a protein that is often 70-90% pure. In addition to protein-isolation applications, MBP provides a high degree of translation and facilitates the proper folding and solubility of the target protein (PMID: 20978971). |
Antibody First Published | Kim et al. Crystal structures of mono- and bi-specific diabodies and reduction of their structural flexibility by introduction of disulfide bridges at the Fv interface. Sci Rep. (2016); 6; 34515. PMID:27682821 |
Note on publication | Describes the crystal structure of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
