Cat. No.
MABL-3994
Application
WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
237mAb
From
Recombinant Antibody
Specificity
The antibody binds specifically to the Tn antigen with a Kd of 140 nM.
Alternative Names
N-acetylgalactosamine-T/S; O-linked N-acetylgalactosamine on a threonine or serine residue
UniProt
Immunogen
Trastuzumab F(ab')2 fragments.
Application Notes
This antibody binds to the idiotype of trastuzumab, effectively mimicking HER2 epitopes. In a murine in vivo model, it elicited anti-HER2 antibodies, offering protection against HER2- positive SK-OV-3 tumor cells (Ladjemi et al., 2011; PMID: 21294885). Additionally, specialized trastuzumab- fused masked forms of this antibody—T-scFv40 + TEV, T-scFv40 + Factor Xa, and T-scFv40 + TEV + Factor Xa—were used to evaluate binding affinity to HER2 via flow cytometry and confocal microscopy (Orozco et al., 2022; PMID: 35799328).
Antibody First Published
Ladjemi et al. Vaccination with human anti-trastuzumab anti-idiotype scFv reverses HER2 immunological tolerance and induces tumor immunity in MMTV.f.huHER2(Fo5) mice. Breast Cancer Res. 2011 Feb 4;13(1):R17. PMID:21294885
Note on publication
Vaccination with human anti-trastuzumab anti-idiotype scFv reverses HER2 immunological tolerance and induces tumor immunity in MMTV.f.huHER2(Fo5) mice.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
