Cat. No.
MABL-3993
Application
cross-competition assay, immunoreactivity, radioiodination, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
I-81C6 (Neuradiab)
From
Recombinant Antibody
Specificity
This antibody is specific for tenascin, which is expressed in restricted locations in normal adult liver, kidney, spleen, and papillary dermis. However, tenascin has been shown to have increased stromal expression in breast carcinomas, gliomas, and prostatic carcinomas. Consequently, this antibody reacts with most human glioma cell lines, xenografts, and biopsy tissue, but not with normal brain tissue.
Alternative Names
Cytotactin; GMEM; GP 150-225; Glioma-associated-extracellular matrix antigen; Hexabrachion; JI; Myotendinous antigen; Neuronectin; Tenascin-C
UniProt
P24821
Immunogen
The glyco-dodecapeptide ERGT(GalNAc)KPPLEELS.
Application Notes
The antibody binds to serine- and threonine linked O-linked N-acetylgalactosamine, a posttranslational glycosylation that is highly characterisitic for Ag104A, an aggressive fibrosarcoma.
Antibody First Published
Schietinger et al. A mutant chaperone converts a wild-type protein into a tumor-specific antigen. Science 2006; 314:304-308 PMID:17038624
Note on publication
Describes how a wild-type transmembrane protein can be transformed into a tumor- specific antigen and how this epitode can be used to generate a specific monoclonal antibody with antitumor activity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
