Cat. No.
MABL-3977
Application
IP
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
Y13-238
From
Recombinant Antibody
Specificity
The antibody reacts with v-H-ras (within amino acids 120-138) and rodent c-H-ras p21′s but not v-K-ras or rodent c-K-ras p21′s. This antibody also recognizes human c-H-ras and c-K-ras p21′s, but not human c-N-ras.
Alternative Names
Ras; p21ras;
UniProt
P78460; O70564
Immunogen
The original antibody was generated by immunizing mice with a synthetic peptide corresponding to amino acids 5-16 (KLVVVGAVGVGK) of a mutated RAS gene encoding Val instead of Gly at position 12 coupled to the carrier protein bovine thyroglobulin (BTG) and to keyhole limpet hemocyanin (KLH).
Application Notes
This antibody was capable of binding G12V and G12C mutant peptides of human ras protein in competition ELISAs (PMID: 3094010). It was reported to cross react with peptides containing amino acids V, A, C, H, I, L, M, Y, T, N, Q at position 12 in an ELISA (PMID: 9440050). This antibody could detect mutant KRAS proteins from cell lysates of human tumor cell lines in a western blot, but did not react with normal KRAS or other KRAS mutants. Immunohistochemical studies showed that DWP-labeled transformed NIH cells and human carcinoma cells contained p21ras is with either Val or Cys at position 12 but not normal or other activated KRAS protein (PMID: 3094010).
Antibody First Published
Carney et al. Monoclonal antibody specific for an activated RAS protein. Proc Natl Acad Sci U S A. 1986 Oct;83(19):7485-9. PMID:3094010
Note on publication
This paper describes the generation and characterization of an antibody that recognizes mutated RAS protein.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
