Cat. No.
MABL-3976
Application
radioimmunodetection, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Dog, Human
Clone No.
TP-3
From
Recombinant Antibody
Specificity
This osteosarcoma-specific antibody binds an epitope on an alkaline phosphatase isoform expressed on both canine and human osteosarcoma cells, but with very limited expression on normal cells and other cancer forms. It also reacts with some other major subtypes of human sarcomas.
Alternative Names
UniProt
Immunogen
Injection of rats subcutaneously with Ha-MuSV transformed NRK cells and screening of sera of individual animals to identify those with the strongest immune response against p21. Spleens were removed from the immunised animals and cell fusion of immune lymphocytes with rat myeloma cell line 210RCY3 Ag 1.2.3 (Y3) was carried out. Supernatants were harvested and assayed for antibodies to p21 by GDP binding assay or immunofluorescence on Ha-SV-transformed MDCK cells, followed by testing for the ability of the antibodies to immunoprecipitate p21.
Application Notes
Y13-238 has been used to immunoprecipitate p21 from human peripheral blood lymphoblasts in order to measure its activation state (Downward 1990). Y13-238 has been used as an anti- ras non-neutralising scFv (Cochet 1998).
Antibody First Published
Furth et al. Monoclonal Antibodies to the p21 Products of the Transforming Gene of Harvey Murine Sarcoma Virus and of the Cellular ras Gene Family JOURNAL OF VIROLOGY, July 1982, p. 294-304 PMID:6287003
Note on publication
Describes the generation of monoclonal antibodies to p21 encoded by Ha-MuSV.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
