Cat. No.
MABL-3974
Application
IB, WB, Block, ELISA, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
OV-TL3
From
Recombinant Antibody
Specificity
The antibody binds to OA3 with an affinity (Kd) of 0.71 nM.
Alternative Names
OA3 TA; Tumour-associated antigen OA3
UniProt
Immunogen
This antibody was raised by immunising BALB/c mice with tumor cells from a human osteosarcoma, TPX, grown as a xenograft in athymic BALB/c nude mice.
Application Notes
In indirect immunofluorescence assays, this antibody has been show to bind to the surface of human osteosarcoma TPX cells, but not to autologous skin fibroblasts, non-sarcomatous tumor cells or peripheral blood lymphocytes (Bruland et al, 1986).In IHC analysis, this antibody has been shown to bind to osteosarcomas, along with other major human sarcoma subtypes, including synovial sarcomas, malignant fibrous histiocytomas, malignant hemangiopericytomas and chondrosarcomas. It does no react with liposarcomas and leio- and rhabdomyosarcomas, or non-sarcomatous malignancies or normal tissue (Bruland et al, 1986). This binding profile matches that of MAb TP-3. A radiolabelled Fab derived from this antibody localises to human osteogenic sarcoma xenografts after intravenous injection (Bruland et al, 1987). In a pilot study, this antibody showed potential as a immunoscintigraphic agent for the early detection of lung metastases in patients with bone sarcoma (Bruland et al, 1994).
Antibody First Published
Bruland et al. New monoclonal antibodies specific for human sarcomas. Int J Cancer. 1986 Jul 15;38(1):27-31. PMID:3522438
Note on publication
Describes the original generation of this antibody, and its characterisation.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
