Cat. No.
MABL-3949
Application
agonist, internalizing, WB, ELISA, FC, IF
Isotype
Engineer antibody
Species Reactivity
Human, Mouse
Clone No.
1C1
From
Recombinant Antibody
Specificity
1C1 binds specifically to the amino-termianal LBD (ligand-binding domain) region of the EphA2 ectodomain (IgG1 KD ~0.8 nM; Fab KD ~140 nM) and not to other related human Eph receptors such as EphA1, and EphA4, or any EphB receptor family members. It is reported to cross react with mouse EphA2.
Alternative Names
EphA2; Epithelial cell kinase; Tyrosine-protein kinase receptor ECK
UniProt
P29317
Immunogen
The original antibody was generated by immunizing mice with human Cyr61.
Application Notes
This antibody can bind recombinant human Cyr61 (hCyr61) in an indirect ELISA. In a Biacore assay the binding affinity of this antibody for human Cyr61 was assessed. The dissociation constant for the original mouse IgG1 version was reported to be Kd=6.2nM. This antibody could also bind human Cyr61 extracted from MDA-MB-231 cells and rhCyr61 in a western blot. It can also be used to detect natural Cyr61 protein in cells using immunofluorescence assay. This antibody further revealed inhibitory effects on MDA-MB-231 cell proliferation, migration, and invasion through downregulation of both AKT and ERK phosphorylation in vitro. It also showed significant efficacy on suppressing primary tumor growth and spontaneous lymph node metastasis in in vivo mouse model (PMID: 22048717). Blocking of Cyr61 using this antibody in Sjogren's syndrome(SS)-like model mice could partially alleviate the inflammation and structure disruption of the SMG in this disease, indicating Cyr61 indeed aggravates inflammatory reaction in vivo.
Antibody First Published
Lin et al. A novel anti-Cyr61 antibody inhibits breast cancer growth and metastasis in vivo. Cancer Immunol Immunother. 2012 May;61(5):677-87. PMID:22048717
Note on publication
Describes the generation of two antibodies against Cyr61 and tests their ability to inhibit breast cancer growth and metastasis in vivo.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
