Cat. No.
MABL-3945
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
SER4
From
Recombinant Antibody
Specificity
The antibody binds specifically to the extracellular domain of human ErbB-2.
Alternative Names
ERBB2; Receptor tyrosine-protein kinase erbB-2; human epidermal growth factor receptor 2
UniProt
P04626
Immunogen
The original antibody was created using phage display from large naïve human scFv libraries.
Application Notes
A5 scFv binds to ErbB3 ECD with a KD of 1.6 × 10−7 M. The ability to bind HER3 was assesed in this antibody using surface plasmon resonance. An anti ErbB2/ErbB3 bispecific antibody made from ML3-9 and A5 clones called the A5-linker-ML3.9 bs-scFv exhibits selective targeting of tumour cells in vitro and in vivo that co-express the two target antigens over tumour cells that express only one target antigen or normal cells that express low levels of both antigens. This study was done using flow cytometry. (Robinson et al, 2008;pmid:18841159).
Antibody First Published
Horak et al. Isolation of scFvs to in vitro produced extracellular domains of EGFR family members. Cancer Biother Radiopharm. (2005); 20(6):603-13. PMID:16398612
Note on publication
Describes the generation and characterization of scFvs against extracellular domain of the EGFR family proteins.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
