Cat. No.
MABL-3943
Application
cytometric bead array, WB, ELISA, FC, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
C7b
From
Recombinant Antibody
Specificity
C7b specifically recognises HER2 at a different epitope to that recognised by Herceptin. HER2 is a protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone.
Alternative Names
NEU; HER-2; HER-2/neu; ERBB2; CD340; MLN 19; NGL; TKR1; erb-b2 receptor tyrosine kinase 2; Receptor tyrosine-protein kinase erbB-2; Metastatic lymph node gene 19 protein; Proto-oncogene Neu; Proto-oncogene c-ErbB-2; Tyrosine kinase-type cell surface receptor HER2
UniProt
P04626
Immunogen
The original antibody was created using phage display from large naïve human scFv libraries.
Application Notes
This antibody is an affinity matured version of the parent scFv antibody C6.5 (Schier et al,1996;pmid: 8918938). The ML3-9 scFv binds to the ErbB2 ECD with a KD of 1.1 nM. Another clone derived from ML3-9 An radioimmunoassay was performed on human serum using this antibody. The ability to bind HER2/neu was assesed in this antibody using surface plasmon resonance. An anti ErbB2/ErbB3 bispecific antibody made from ML3-9 and A5 clones called the A5-linker-ML3.9 bs-scFv exhibits selective targeting of tumour cells in vitro and in vivo that co-express the two target antigens over tumour cells that express only one target antigen or normal cells that express low levels of both antigens. This study was done using flow cytometry (Robinson et al, 2008;pmid:18841159).
Antibody First Published
Schier et al. Isolation of picomolar affinity anti-c-erbB-2 single-chain Fv by molecular evolution of the complementarity determining regions in the center of the antibody binding site. J Mol Biol. 1996 Nov 8;263(4):551-67. PMID:8918938
Note on publication
Describes the affinity maturation of the parental antibody to obtain a high affinity binder.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
