Cat. No | MBA-046 |
Name | Rabbit anti-Somatostatin Antibody |
中文名字 | 兔抗生长抑素抗体 |
Description | The antibody has a proven strong Biotin-Streptavidin/HRP staining at a 1/1000-1/2000 dilution in rat hypothalamus (median eminence). The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 106M. |
Clonality | Polyclonal |
Isotype | IgG |
Host | Rabbit |
Quantity/Volume | 50ul/100 µL |
State | Liquid Whole Serum/antibody |
Reacts With | Alligator, Anuran (Frog), Bat, Bird, Buffalo (Bubalus Bubalis), Bullfrog, Cat, Chick, Chicken, Dermophis Mexicanus Mexican Caecilian (Amphibian ), Dog, Duck, Fish, Fowl, Frog (Xenopus Laevis), Guinea Pig, Hamster, Human, Lizard, Malayan Pangolin (Sunda Pangolin), Manducta Sexta (Moth), Monkey, Mouse, Pig, Pigeon, Quail, Rabbit, Raccoon, Rana Pipiens (Leopard Frog), Rat, Scorpaena Porcus (Fish), Sea Bass (Fish), Sheep, Snail, Snake, Sparus Aurata (Fish), Starfish, Sting Ray, Turtle, Zebrafish |
Preabsorption Control | Available at Sigma Aldrich, Catalog # S9129 |
Alternate Names | Growth hormone release-inhibiting factor; Somatostatin 14; Somatostatin 28; prepro-somatostatin; SMST; anti-somatostatin |
RRID | AB_572264 |
Immunogen | This information is proprietary to Mabioway |
Gene Symbol | SST |
Entrez Gene ID | Entrez Gene: 102569049 Alligator |
NCBI Gene Aliases | / |
Sequence | P61278 |
APPLICATION | |
Quality Control | The antibody produces significant indirect immunofluorescent staining at a 1/400–1/800 dilution and significant Biotin-avidin/HRP staining at a 1/1,000–1/2,000 dilution in rat hypothalamus (median eminence). The specificity of the antiserum was examined by soluble preadsorption with the peptides at a final concentration of 106 M. Somatostatin immunolabeling was completely abolished by preadsorption with somatostatin, somatostatin 25, and somatostatin 28. Preadsorption with the following peptides resulted in no reduction of immunostaining: substance P, amylin, glucagon, insulin, neuropeptide Y, and VIP. |
Tissue | Rat hypothalamus (median eminence) |
Perfusion Fixation | • Fixation: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20-30 min |
Absorption Control | |
Sections | 10 µm cryostat |
Tissue Incubation | 18–24 hours at 2°–8°C |
Detection System | Use Cy3 or Bn-AV/HRP reagents at dilutions recommended by manufacturers. |
Suggested Dilution | 1/1,000–1/2,000 in PBS/0.3% Triton X-100 – Bn-AV/HRP immunohistochemistry |
NOTES | |
Special Instructions | It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested. |
Concentration | Not applicable. Antibody concentration is only relevant for purified antibodies. |
Storage | After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles |
