Rabbit anti-Neurotensin Antibody

Name

Rabbit anti-Neurotensin Antibody

中文名字

兔抗神经降压素抗体

Description

The Neurotensin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat amygdala using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/4000-1/8000 in PBS/0.3% Triton x-100 – Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment with 10 µg of Neurotensin per 1 mL of diluted antibody.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Cat, Dog, Frog, Human, Monkey, Mouse, Pigeon, Rat, Turtle

Preabsorption Control

/

Alternate Names

neuromedin N; Preproneurotensin; pro-neurotensin, anti-neurotensin

RRID

AB_572254

Immunogen

This information is proprietary to Mabioway

Gene Symbol

NTS

Entrez Gene ID

Entrez Gene: 101095835    Cat
Entrez Gene: 611687         Dog
Entrez Gene: 100485937    Frog
Entrez Gene: 4922             Human
Entrez Gene: 700189         Monkey
Entrez Gene: 67405           Mouse
Entrez Gene: 102087679    Pigeon
Entrez Gene: 299757         Rat
Entrez Gene:102446489    Turtle

NCBI Gene Aliases

/

Sequence

39379-15-2

APPLICATION

Quality Control

The Neurotensin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat amygdala using indirect immunofluorescent and biotin/avidin-HRP techniques. Staining is completely eliminated by pretreatment with 10 µg of Neurotensin per 1 mL of diluted antibody.

Tissue

Rat amygdala

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20–30 min.
• Post Fixation: 1.5 hr at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.

Absorption Control

Neurotensin 10 ug/mL diluted serum

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

18–24 hours at 2°–8°C

Detection System

Use Cy3 or Bn/Av-HRP according to manufacturers’ directions.

Suggested Dilution

1/4,000–1/8,000 in PBS/0.3% Triton x-100 – Bn/Av-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

After reconstitution, use immediately or refrigerate at 2º–8ºC up to 2 days. For long-term storage, aliquot antibody and freeze at -15°C or lower. Avoid repeated freeze/thaw cycles