Rabbit anti-Neurokinin 3 Receptor Antibody

Name

Rabbit anti-Neurokinin 3 Receptor Antibody

中文名字

兔抗神经激肽3受体抗体

Description

The NK3R antiserum was quality control tested using standard immunohistochemical methods in rat hypothalamus using biotin/avidin-HRP techniques.
Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Western blot analysis of crude rat brain homogenate demonstrates two immunoreactive bands of approximately 80 and 115 kD.
Tissue staining is completely eliminated by pretreatment of the diluted antibody with 25 mg of rat NK3R peptide residues 438-452.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Rat

Preabsorption Control

/

Alternate Names

Tachykinin receptor 3; TACR3; NK-3 receptor; NKR, Neurokinin B receptor; Neuromedin-K receptor; TAC3R; Tachikin 3; Nmkr, anti-NK-3

RRID

AB_10013881

Immunogen

This information is proprietary to Mabioway

Gene Symbol

 Tacr3, 24808

Entrez Gene ID

NCBI Gene Aliases

/

Sequence

P29371

APPLICATION

Quality Control

The NK3R antiserum was quality control tested using standard immunohistochemical methods in rat hypothalamus using biotin/avidin-HRP techniques. Specificity of the antiserum was demonstrated by soluble preadsorption and western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with 25 mg of rat NK3R peptide residues (438–452). Western blot analysis of crude rat brain homogenate demonstrates two immunoreactive bands of approximately 80 and 115 kD. Due to the difficulty in running westerns with this antibody, ImmunoStar does not support or warrant the western blot application. The western information is included as specificity information only.

Tissue

Rat brain - hypothalamus

Perfusion Fixation

• Fixative: 4% paraformaldehyde in 0.1 M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4.
• Note: Paraformaldehyde is a necessary component in fixation. If needed, low levels of glutaraldehyde (0.1–0.3%) may be used in conjunction with paraformaldehyde.

Absorption Control

Rat NK3R (438–452) 25 ug/mL diluted antibody completely eliminates immunolabeling

Sections

10 µm cryostat or 50 µm vibratome

Tissue Incubation

48 hours at 2°–8°C

Detection System

 Bn/AV-HRP at dilutions recommended by the manufacturers.

Suggested Dilution

1/1,000–1/2,000 in PBS/0.3% Triton X-100 for Bn/AV-HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

Store at 2°–8°C until expiration date.