Rabbit anti-5-HT (Serotonin) 1A Receptor Antibody

Name

Rabbit anti-5-HT (Serotonin) 1A Receptor Antibody

中文名字

兔抗-5-HT 1A受体抗体，兔抗血清素抗体

Description

The histochemical antibody for 5-HT1A receptor is generated in a Rabbit against synthetic peptide sequence corresponding to amino acids 294-312 of the rat 5-HT1A receptor. The antiserum is provided as 100 µl of affinity purified serum containing 1% BSA. Controls: The ImmunoStar 5-HT1A receptor antiserum was quality control tested using standard immunohistochemical methods.
The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/200 – 1/300 in PBS – Bn/Av-HRP detection. Intensification methods such as nickel will approximately double the dilution factor as recommended.
The antibody was characterized by immunohistochemistry and Western blot. Western blot showed a single band of approximately 45 kD using a dilution of 1/100. Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only.  Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography.
BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT1A receptors.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid

Reacts With

Guinea Pig, Human, Monkey, Mouse, Rat

Preabsorption Control

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Alternate Names

5-hydroxytryptamine receptor 1A; ADRB2RL1; ADRBRL1; RAT5HT1A, 5-hydroxytryptamine (serotonin) receptor 1A, G protein-coupled, anti-5-HT 1A

RRID

AB_572210

Immunogen

This information is proprietary to Mabioway

Gene Symbol

Htr1a

Entrez Gene ID

  Entrez Gene: 100301563  Guinea Pig
  Entrez Gene: 3350  Human
  Entrez Gene: 694991  Monkey
  Entrez Gene: 15550  Mouse
  Entrez Gene: 24473  Rat

NCBI Gene Aliases

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sequence

>sp|P08908|5HT1A_HUMAN 5-hydroxytryptamine receptor 1A 

APPLICATION

Quality Control

The 5-HT1A receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Intensification methods such as nickel will approximately double the dilution factor as recommended. The antibody was characterized by immunohistochemistry and western blot. Western blot showed a single band of approximately 45 kD. Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only. Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography.

Tissue

Rat cortex, arcuate and hippocampus

Perfusion Fixation

• Fixative: 4% paraformaldehyde/0.05% glutaraldehyde in 0.1M Phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, pH 7.4.

Absorption Control

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Sections

50 µm vibratome. Cryosections are not recommended as it may disrupt the membrane. This antibody does not work with paraffin embedded tissues.

Tissue Incubation

48 hours at 2°–8° C.

Detection System

Use Bn/AV-HRP at dilutions recommended by the manufacturer. For immunofluorescence stain wash in 1% sodium borohydride after the wash step following fixation in order to quench aldehyde sites and aid in suppressing background. See instructions online for western blot suggestions.

Suggested Dilution

1/200 –1/300 in PBS - Bn/Av-HRP detection. 1/100 or greater in Western Blot. Note: Use of Triton X-100 or other detergents is not recommended. If necessary, use only 0.03% T-X100 in the block only. No more detergent thereafter. Further penetration enhancement may be achieved by subsequent 5 min washes in 10, 25, 40, and 50% ethanol in PBS, followed by reversing wash order back down to PBS.

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

Store at 2°–8°C until expiration date.