Rabbit anti-5-HIAA (5-Hydroxyindoleacetic Acid) Antibody

Name

Rabbit anti-5-HIAA (5-Hydroxyindoleacetic Acid) Antibody

中文名字

兔抗5-HIAA抗体， 兔抗5-羟基吲哚乙酸抗体

Description

The antibody produces moderate labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a maximum label. Recommended dilution of the antiserum is 1/4000-1/8000 for biotin-streptavidin/HRP technique.
The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTP. The antiserum was also tested by pre-adsorption at 25 µg/mL with various BSA conjugates. While pre-adsorption with 5-HIAA conjugate completely eliminates immunolabeling, pre-adsorption with conjugates of 5-HT,5-HTP and dopamine had no effect on staining intensity or distribution of stain.

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Quantity/Volume

50ul/100 µL

State

Liquid Whole Serum/antibody

Reacts With

Rat, Tritonia Diomedea (Sea Slug)，Mollusca

Preabsorption Control

/

Alternate Names

anti-5-HIAA

RRID

AB_572208

Immunogen

This information is proprietary to Mainway

Gene Symbol

SLC6A4

Entrez Gene ID

6532

NCBI Gene Aliases

5-HTT,5-HTTLPR,5HTT, HTT,OCD1,SERT,hSER

sequence

54-16-0

APPLICATION

Quality Control

The antibody produces significant labeling of raphe neurons in normal rat. In rats whose serotonergic system has been activated, staining intensity is increased to a significant label. Recommended dilutions of the antiserum are 1/4,000–1/8,000 for indirect immunofluorescence and for biotin-streptavidin/HRP technique. The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Schipper and Tilders, 1983). Results showed that the 5-HIAA antibody dose dependently stained 5-HIAA but did not stain any concentration of 5-HT or 5-HTP. The antiserum was also tested by preadsorption at 25 mg/mL with various BSA conjugates. While preadsorption with 5-HIAA conjugate completely eliminates immunolabeling, preadsorption with conjugates of 5-HT, 5-HTP and dopamine had no effect on staining intensity or distribution of stain.

Tissue

Rat dorsal and median raphe neuronal cell bodies. Serotonergic system may be activated by salt loading which is achieved by 2% NaCl placed in drinking water for 48 hours prior to perfusion.

Perfusion Fixation

• Fixation: 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4; 500 mL over 20 min.
• Post Fixation: 1.5 hour at 4°C in 4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4.
• Note: Paraformaldehyde is a necessary component of fixation for this antiserum. If needed for other applications, glutaraldehyde may be used at low levels (0.1–0.3%) in conjunction with paraformaldehyde.

Absorption Control

Sections

10um cryostat or 50um vibratome

Tissue Incubation

18–24 hours at 2°–8°C.

Detection System

Use IF or Bn-AV/HRP reagents at dilutions recommended by the manufacturers

Suggested Dilution

1/4,000–1/8,000 in PBS/0.3% Triton X-100 – Bn-AV/HRP immunohistochemistry

NOTES

Special Instructions

It is recommended that the researcher perform a primary antibody dilution series using our dilution recommendations as a guideline. Note that a change in the fixation or buffering system from our protocol may change the configuration of the protein which could alter the reactivity with the tissue tested.

Concentration

Not applicable. Antibody concentration is only relevant for purified antibodies.

Storage

Store at-20°C until expiration date.