Anti-CD25 [7D4](MABL-3493)

Cat. No.

MABL-3493

Name

Anti-CD25 [7D4](MABL-3493)

Clone No.

7D4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

depleting, FC

Species Reactivity

Mouse

Basic Information

Specificity

7D4 recognizes mouse CD25 at an epitope   comprising amino acid 184 to 194 (REHHRFLASEE). This epitope is distinct from   the IL-2 binding site and does not block binding of IL-2 to CD25. Murine CD25   is a 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is   expressed by early progenitors of the T and B lineage as well as by activated   mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low   affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and   CD132 (common gamma chain) to form the high affinity IL-2 receptor.

Alternative Name

IL-2-RA; Interleukin-2 receptor subunit alpha; IL-2 receptor subunit alpha; IL-2R subunit alpha; IL2-RA; p55

UniProt

P01590

Immunogen

The original rat IgM version of this antibody   was raised by immunizing Lewis rats with HT2 cells in complete Freund's   adjuvant via a intraperitoneal injection and subsequent generation of   hybridomas.

Application Notes

7D4 is a non-IL-2 blocking anti-CD25   antibody that has been widely used to identify CD25-positive cells via flow   cytometry (Kohyama et al., 2012; pmid: 22399592), for instance, to analyse   Lymph node cells from BALB/c mice that were untreated or treated with 0.25 mg   PC61 (Onizuka et al., 1999; pmid: 10397255) or to characterize   brain-infiltrating immune cells (Maes et al., 2013). Although 7D4 does not   inhibit IL-2 binding itself, it shows synergistic qualities with a cancer vaccine; namely, 7D4 with a cancer vaccine boosted the vaccine-induced   anti-tumour response. Furthermore, this antibody was shown to be able to   induce inactivation of regulatory T cells (Treg); the effect was particularly   significant when this clone was used synergistically with another anti-CD25   clone - PC61 (Ab01107) (Kohm et al., 2006; pmid: 16517695; McNeill et al.,   2007; pmid: 17212768). 7D4, when turned into a mouse IgG2a format, exhibits   depleting qualities against Treg cells and can be used to remove Treg   lymphocytes from the tumoral environment without blocking CD25 (IL-2 pathway)   on T effector cells (Solomon et al., 2020). 

Antibody First   Published

Malek et al. Identification and initial characterization of a rat monoclonal antibody reactive with the murine interleukin 2 receptor-ligand complex Proc Natl Acad Sci U S A. 1983 Sep;80(18):5694-8. doi: 10.1073/pnas.80.18.5694.PMID:6412230

Note on publication

Describes the generation and characterization of the antibody 7D4.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.