Anti-TIGIT [4D4](MABL-3491)

Cat. No.

MABL-3491

Name

Anti-TIGIT [4D4](MABL-3491)

Clone No.

4D4

From

Recombinant Antibody

Isotype

Engineer antibody

Application

agonist, FC

Species Reactivity

Mouse

Basic Information

Specificity

4D4 specifically recognises TIGIT.

Alternative Name

VSIG9; T Cell Immunoreceptor With Ig And   ITIM Domains; V-Set And Immunoglobulin Domain Containing Protein 9; V-Set And Transmembrane Domain Containing Protein 3; VSTM3; T Cell Immunoreceptor With   Ig And ITIM Domains; Washington University Cell Adhesion Molecule; WUCAM

UniProt

P86176

Immunogen

Armenian hamsters were immunized with   recombinant mouse TIGIT tetramers by a combination of s.c. and food pad   immunization and booster injections. Draining lymph nodes were fused with   Sp2/0-Ag14 cells, selected in HAT (hypoxanthine/aminopterin/thymidine) medium   and supernatants were screened for specificity by ELISA and flow cytometry   using TIGIT-transfectants.

Application Notes

4D4 is an agonistic anti-TIGIT antibody, and addition of plate bound 4D4 to anti- CD3/anti-CD28 stimulated T cells was shown to inhibit their proliferation (Joller 2011). Further, 4D4 has been   used in order to identify genes that are downstream of TIGIT signaling in   Tregs, as TIGIT engagement might modulate the Treg phenotype in tumor tissue   (Liu 2016).

Antibody First   Published

Joller et al. Cutting edge: TIGIT has T cell-intrinsic inhibitory functions. J Immunol. 2011 Feb 1;186(3):1338-42   PMID:21199897

Note on publication

Describes the generation of anti-TIGIT monoclonal antibodies and their use to analyse the function of TIGIT in   cells.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.