Anti-CD45R [RA3-6B2](MABL-3477)

Cat. No.

MABL-3477

Name

Anti-CD45R [RA3-6B2](MABL-3477)

Clone No.

RA3-6B2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WV, FC, IF, IHC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine CD45R   antigen which is expressed on B lymphocytes throughout their development from early pro-B stages onwards and is down-regulated upon terminal   differentiation to plasma cells. Apart from B cells, CD45R is expressed on a small subset of dendritic cells (plasmacytoid dendritic cells). The CD45R mAb   clone RA3-6B2 specifically recognizes the exon A–restricted isoform of mouse CD45.

Alternative Name

B220; CD45; CD45 antigen; Cluster designation 45R; L-CA; Leukocyte common antigen; Ly-5; lymphocyte antigen 5;   lymphocyte common antigen; Lyt-4; protein tyrosine phosphatase, receptor   type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200   leukocyte common antigen

UniProt

P06800

Immunogen

This antibody was raised in Lou-M rats using   a popliteal lymph node immunisation and fusion protocol as described by   Martiniello et al (1994)

Application Notes

This antibody has been used in FACS analyses, immunoprecipitation, immunoblotting, and immunohistochemistry in   various studies, such as to understand the role of PD-1 in maintaining islet-   specific tolerance in type 1 diabetes (Martinov et al, 2016), to investigate   how Staphylococcus aureus enterotoxins spread systemically and trigger   inflammatory cascades (Svedova et al, 2016), to highlight the physiologic   importance of cell-type specific balancing of AhR/AhRR expression in response   to microbial, nutritional and other environmental stimuli (Brandstätter et   al, 2016), and to examine the role of FBXW7 in suppression of cancer   metastasis in a non-cell-autonomous manner (Yumimoto et al, 2015).

Antibody First   Published

Stephen Hurst et al. New IL-17 family members promote Th1 or Th2 responses in the lung: in vivo function of the   novel cytokine IL-25. J Immunol. 2002 Jul 1;169(1):443-53. PMID:12077275

Note on publication

Describe the use of this antibody for FACS analysis to demonstrate the function of the novel cytokine IL-25 in the lung.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.