Anti-PD-L1 [10F.9G2](MABL-3462)

Cat. No.

MABL-3462

Name

Anti-PD-L1 [10F.9G2](MABL-3462)

Clone No.

10F.9G2

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Blocking, ELISA, FC, IHC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine PD-L1.

Alternative Name

CD274; PDL1; PD L1; Programmed cell death 1 ligand 1; PDCD1 ligand 1; Programmed death ligand 1; B7 homolog 1; B7-H1; 10F9G2; 10F 9G2

UniProt

Q9EP73

Immunogen

This antibody was raised by immunising Lewis rats with murine PD-L1 cDNA and murine PD- L1 CHO transfectants.

Application Notes

The specificity of this antibody has been confirmed in ELISA analysis (Eppihimer et al, 2002). This antibody reacts   specifically with mPD-L1-transfected cells in flow cytometry, and has been used to assess microvascular endothelial cells PD-L1 expression (Eppihimer et al, 2002). When administered to mice, a radiolabelled version of this antibody has been used to quantify PD-L1 expression in vivo, by measuring antibody accumulation in different tissues (Eppihimer et al, 2002). This antibody has also been used in immunohistochemical analysis of PD-L1   expression in frozen tissue sections of murine brain (Eppihimer et al, 2002)   and murine hearts (Rodig et al, 2003). This antibody has been shown to block   the binding of PD-L1 to both PD-1 and B7-1 (Paterson et al, 2011). This   antibody precipitates diabetes in NOD mice and in adoptive transfer models of   CD4+ and CD8+ T cell-driven diabetes, and accelerates diabetes in recipients of T cells from diabetic and prediabetic mice (Paterson et al, 2011).

Antibody First   Published

Eppihimer et al. Expression and Regulation of the PD-L1 Immunoinhibitory Molecule on Microvascular Endothelial Cells Microcirculation. 2002 Apr;9(2):133-45. PMID:11932780

Note on publication

Describes the original generation of this antibody, and its use in ELISA, flow cytometry and immunohistochemistry.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.