Anti-Tim-1 [3B3](MABL-3459)

Cat. No.

MABL-3459

Name

Anti-Tim-1 [3B3](MABL-3459)

Clone No.

3B3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

agonism, FC, IF, IHC

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine TIM-1, a member of the T cell immunoglobulin domain, mucin-like domain (TIM) gene family. TIM-1 has been found on activated CD4+ T cells, mast cells, and a   subset of B cells. TIM-1 is involved in renal injury, tissue homeostasis, and T cell costimulation, and promotes the differentiation of Th2 cells and the   production of IL-4. TIM-1 can bind several ligands, including Hepatitis A   virus, phosphatidylserine, TIM4, IgA, as well as itself.

Alternative Name

CD365; HAVcr-1; HAVCR1; Hepatitis A virus cellular receptor 1 homolog; Kidney injury molecule 1; KIM-1; T cell   immunoglobulin and mucin domain-containing protein 1; T cell membrane protein 1; T-cell immunoglobulin and mucin domain containing 1; T-cell immunoglobulin mucin receptor 1; TIM-1; TIM1; TIMD-1

UniProt

Q5QNS5

Immunogen

This antibody was raised by immunising   female Lewis strain rats (Harlan Sprague-Dawley) subcutaneously with mouse   TIM-1–Ig in complete Freund's adjuvant (CFA), followed by multiple 'boosting'   with mouse TIM-1–Ig in PBS.

Application Notes

This antibody has been used in multiple FACS analyses, such as to determine if specific B cell subsets or if B   cell-derived interleukin-10 contributes to tolerance (Lai et al, 2015), to   test if blocking several checkpoint receptors boosts anti-tumor immunity in a low-dose, lympho-depleting whole body irradiation model (Jing et al, 2015),   and to study the functions of kidney pericytes in vascular stability   (Schrimpf et al, 2012). This antibody has also been used in immunohistochemistry   to investigate the distinct role of matrix metalloproteinase-3 in TIM-1 shedding by kidney proximal tubular epithelial cells (Lim et al. 2012), and   to demonstrate how Bβ(15-42) attenuates the effect of ischemia-reperfusion   injury in renal transplantation (Sörensen et al, 2011). In addition, in vivo applications of this agonistic anti-TIM-1 antibody (clone 3B3) has been shown, for instance, to heighten T cell activation and prevent the   development of respiratory tract tolerance in a Th2-driven model of asthma   (Umetsu et al, 2005), to increase the frequency of antigen-specific T cells,   the production of the proinflammatory cytokines IFN-γ and IL-17, and thus the   severity of experimental autoimmune encephalomyelitis (Sheng et al, 2007), as well as to deprogram Tregs and prevent transplant tolerance in mice (Degauque et al, 2008). 

Antibody First   Published

Umetsu Sarah et al. TIM-1 induces T cell activation and inhibits the development of peripheral tolerance. Nat Immunol. 2005 May;6(5):447-54. PMID:15793575

Note on publication

Describe the original generation of this antibody and its use in FACS analysis and in a TCR-transgenic DO11.10 mouse   model to demonstrate that TIM-1 induces T cell activation and inhibits the development of peripheral tolerance.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.