Anti-CD25 [PC-61.5.3](MABL-3450)

Cat. No.

MABL-3450

Name

Anti-CD25 [PC-61.5.3](MABL-3450)

Clone No.

 PC-61.5.3

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Depletion, functional assays, WB, ELISA, FC, IF

Species Reactivity

Mouse

Basic Information

Specificity

This antibody is specific for murine CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is   expressed by early progenitors of the T and B lineage as well as by activated   mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low   affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and   CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding   of IL-2 to both the high and low affinity classes of IL-2 receptor is   inhibited by the PC61.5 antibody.

Alternative Name

IL2-RA; IL-2 receptor subunit alpha;L-2R alpha chain; IL-2R subunit alpha; Interleukin-2 receptor subunit alpha; p55; p55 chain; PC61; PC6153

UniProt

P01590

Immunogen

This antibody was raised by immunising rats   with the B6.1 mouse cytotoxic T cell line, followed by fusing spleen cells   with P3X63Ag8.653 myeloma cells.

Application Notes

This antibody has been used in numerous FACS analyses, for instance, to demonstrate that glycolysis and glutaminolysis cooperatively control T cell function by limiting metabolite supply to N- glycosylation (Araujo et al, 2017), to suggest that IL-2C could be a   potential therapeutic method to alleviate excessive inflammation and maintain   blood vessel stability after traumatic brain injury (Gao et al, 2017), and to   evaluate how alternative splicing of MALT1 controls signalling and activation   of CD4(+) T cells (Meininger e al, 2016). This antibody has also been used in   immunofluorescence as part of the DNA Aβ42 immunization studies in mice   (Lambracht-Washington et al, 2015), in ELISA assays to investigate the   dynamics of T cell receptor (TCR)-dependent signaling networks (Brockmeyer et   al, 2011), and in Western Blot to determine the effects of increased p300   expression on glucocorticoid receptor (GR)-T-cell-receptor (TCR) crosstalk   between thymocytes (Yu et at, 2002).

Antibody First   Published

Trowbridge et al. Murine cell surface transferrin receptor: studies with an anti-receptor monoclonal antibody. J Cell Physiol. 1982 Sep;112(3):403-10. PMID:6290505

Note on publication

Describe the original generation of this antibody and its subsequent characterisation by FACS analysis and in vitro biochemical assays.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified   antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity   purified

Buffer

PBS with 0.02% Proclin   300.

Concentration

1 mg/ml.

Storage   Recommendation

Store at 4⁰C for up to 3   months. For longer storage, aliquot and store at - 20⁰C.