Key features and details | |
Cat. No. | MABL-664 |
Name | Anti-CD10 mAbs |
Clone No. | AFD- NL-1 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody reacts with human CD10. CD10 is a widely-expressed cell-surface metalloprotease, responsible for the cleavage of multiple peptide hormones at target tissues. CD10 is also a common marker for acute lymphocytic leukaemia. |
Alternative Name | Neprilysin; Cluster of Differentiation 10; Membrane metallo- endopeptidase; MME; Neutral endopeptidase 24.11; Neutral endopeptidase; NEP; Common acute lymphocytic leukaemia antigen; CALLA; Skin fibroblast elastase; SFE; CMT2T; SCA43; Atriopeptidase; Enkephalinase |
UniProt | P08473 |
Immunogen | BALB/c mice were immunised with acute lymphocytic leukaemia cells from a human leukaemia patient. Splenocytes were obtained from immunised mice and fused with MOPC-21 NS/1 myeloma cells to generate a hybridoma. |
Application Notes | NL-1 was shown to react with several leukaemic cell lines using immune adherence assays (Ueda et al, 1982). These authors also confirmed that NL-1 is specific for CD10 using IP. |
Antibody First Published | Ueda et al. Serological analysis of cell surface antigens of null cell acute lymphocytic leukemia by mouse monoclonal antibodies. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4386-90. PMID:6956869 |
Note on publication | Describes the generation and characterisation of a panel of mAbs against various leukaemic cell lines with the goal of using this panel of mAbs to define blood antigen systems. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
