Key features and details | |
Cat. No. | MABL-601 |
Name | Anti-Capsid protein VP1 mAbs |
Clone No. | AFD- Mab14 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | cryoEM, crystallography, HI, NTRL, RIA, ELISA |
Species Reactivity | canine parvovirus |
Basic Information | |
Specificity | This antibody is specific for Site A of CPV-2's capsid. |
Alternative Name | Coat protein VP1; CPV-2; Fab 14; Antibody 14; ABCD_AD535 |
UniProt | P17455 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with purified Capsids of CPV and FPV which were prepared from infected feline cells. |
Application Notes | The original version of this antibody (IgG2a) was raised and tested against CPA, FPV, and MEV and was found to inhibit and neutralize CPV-a and -b in neutralization and HI assays (Parrish et al., 1982; PMID: 6180709). The original version of this antibody was used in RIA and HI assays. In the HI test, it was found to inhibit CPV-a;-b;-c-;-d; and -e, and in the RIA, it was found to react with CPV-d variants 6, 12, and 8 (Parrish and Carmichael, 1983; PMID: 6194613). The Fab version of this antibody was used in a cryoEM analysis of capsid: Fab complexes, and its Kd was measured via ELISA as 3.4 × 10-9 M (Nelson et al., 2007; PMID: 17217977). The Fab version of this antibody was crystallized, and its structaure was determined (Hafenstein et al., 2009; PMID: 19321620). |
Antibody First Published | Parrish et al. Antigenic relationships between canine parvovirus type 2, feline panleukopenia virus and mink enteritis virus using conventional antisera and monoclonal antibodies. Arch Virol. 1982;72(4):267-78. doi: 10.1007/BF01315223. PMID:6180709 |
Note on publication | The original publication investigates the antigenic relationships between canine parvovirus type 2 (CPV), feline panleukopenia virus (FPV), and mink enteritis virus (MEV) using conventional antisera and monoclonal antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
